Thesis (Ph.D) - University of Birmingham, School of Chemical Engineering, Faculty of Engineering.
|Statement||by Dipankar Dey.|
Hence, information about gas bubble sizes in micro-bubble aerated and stirred bioreactors filled with the culture medium of choice, as well as their behavior at varying process conditions, are crucial in order to gain insights regarding cell stress based on cell/bubble interactions. 2. Materials and methods Vessel configuration and mediumCited by: Unfortunately the hydrodynamic conditions lead to cell damage due to cell bursting at the surface () and not cell-bubble interactions within the bulk fluid (26, 52, 53). Bubbles adhere to the. During the disengagement process, smaller bubbles travel upward slower, whereas, t = M (t = 3 s for plane 3 and t = 4 s for plane 5) corresponds to the last stage of the gas disengagement of the smallest bubbles. Accordingly, during the DGD process and after shutting off the gas supply, the gas holdup began to descent in the aerated coaxial Cited by: However, since it may lead to larger bubbles, I I i Bubble i Liquid 1" b Bubbl Bubble ~/~ Liquid ~. Figure 3 Sketches showing two types of cell-bubble interactions which are most likely to damage cells. (a) Ceils near the bubble in- terface experience large shear stresses during breakup when the bubble surface is collapsing at very high speed.
Ningning Ma, Jeffery J. Chalmers, John G. Auniņš, Weichang Zhou, Liangzhi Xie, Quantitative Studies of Cell‐Bubble Interactions and Cell Damage at Different Pluronic F‐68 and Cell Concentrations, Biotechnology Progress, /bp, 20, 4, (), (). Microscopic visualisation of insect cell-bubble interactions — II: The bubble film and bubble rupture. Biotech Prog 7, CrossRef Google Scholar Cherry, R S & Hulle, C T, The gas sparging of culture broth causes damage to suspended animal cells. However, despite this, sparged bioreactors remain the preferred means of cell culture because sparging is a robust method of supplying oxygen, especially on a large scale. This article examines the underlying mechanisms involved in bubble-associated cell damage and the methods available for controlling such damage. Pluronic F‐68 is a widely used protective agent in sparged animal cell bioreactors. In this study, the attachment‐independent Spodoptera frugiperda Sf9 insect cell line was used to explore the mechanism of this protective effect and the nature of cell damage in sparged bioreactors. First, bubble incorporation via cavitation or vortexing was induced by increasing the agitation rate in a.
We determined: (1) the number of cells killed as a result of a single, ‐mm bubble rupture; (2) the number and viability of cells in the upward jet that results when a bubble ruptures; (3) the number of cells on the bubble film; and (4) the fate of cells attached to the bubble film after film rupture. Dey D () Cell–bubble interactions during bubble disengagement in aerated bioreactors. PhD Thesis, The University of Birmingham, UK de Zengotita VM, Schmelzer AE, Miller WM () Characterisation of hybridoma cell responses to elevated pCO 2 and osmolality: intracellular pH, cell size, apoptosis and metabolism. Orton DR, Wang DIC () Fluorescent visualization of cell death in bubble aerated bioreactors. Cell culture engineering III, Engineering Foundation, 2–7 Feb Google Scholar Ramirez OT, Mutharasan R () The role of the plasma membrane fluidity on the shear sensitivity of hybridoma growth under hydrodynamic stress. Fig. 4 Zones of potential bubble–cell interactions that may cause cell damage (Oh et al. ) Fig. 5 Increasing gross vortexing in an unbafﬂed 2 L animal cell bioreactor: (a) at rpm.